|Offered||Study Year 1, Semester 2|
|Course Coordinator||Gruber, Ardina (Dr)||Gruber@ntu.edu.sg||6316 2926|
|Contact hours||Lectures: 24, Tutorials: 13, Laboratories: 12|
|Approved for delivery from|
|Last revised||28 Nov 2022, 14:03|
This course aims to introduce the structural, biophysical and chemical principles of key biological macromolecules found in living organisms. You will use your understanding of the underlying molecular bases and functions of the macromolecules involved in various biochemical reactions to solve problems related to nucleic acids, proteins, carbohydrates, lipids and vitamins. You will also develop the ability to conduct biochemical experiments in the lab and design new molecules based on knowledge of the protein data bank.
Upon successfully completing this course, you should be able to:
Building blocks of macromolecules (covalent and non-covalent bonding, free energy, acids, bases, buffers)
Primary, secondary and tertiary structures of nucleic acids (DNA, RNA), proteins, polymers (carbohydrates, lipids)
Modifications of nucleic acid and proteins (e.g. spontaneous, chemical, genetic, post-translational)
Folding of proteins, quaternary structure of proteins (myoglobin, hemoglobin)
Carbohydrates (tautomers, stereochemistry, ring structures, saccharides)
Lipids (triglycerides, phospholipids, membrane transfer)
Vitamins (solubility, deficiency and disease, dietary issues)
How to use Pymol molecular visualization software
Accessing biological databases such as Protein Data Bank (PDB)
|Component||Course ILOs tested||SBS Graduate Attributes tested||Weighting||Team / Individual||Assessment Rubrics|
|Written Report||1, 2, 3, 5, 6, 7, 8, 9||1. a, b |
2. a, b, e, g
3. a, b, e
5. a, b, c, d, e
7. a, b, c
|50||individual||See Appendix for rubric|
|Examination (2 hours)|
|Multiple Choice Questions||1, 2, 4, 5, 6, 8, 10, 11||1. a, b |
2. a, e
3. a, b, e
5. a, c, d, e
These are the relevant SBS Graduate Attributes.
1. Recognize the relationship and complexity between structure and function of all forms of life, resulting from an academically rigorous in-depth understanding of biological concepts
a. Possess a conceptual framework that identifies the relationships between the major domains in the field of biology.
b. Explain the relationship between structure and function of all forms of life at the molecular level
2. Critically evaluate and analyze biological information by applying the knowledge, scientific methods and technical skills associated with the discipline
a. Identify the assumptions behind scientific problems and issues
b. Create and evaluate hypotheses
e. Analyze the validity of qualitative and quantitative scientific data
g. Evaluate the results of their own experiments and decide on the next step
3. Develop and communicate biological ideas and concepts relevant in everyday life for the benefit of society
a. Simplify and explain scientific concepts and results of experiments to a non-biologist (avoiding jargon)
b. Display and explain scientific results clearly and persuasively to peers both verbally and in writing (includes the ability to graph data appropriately and accurately).
e. Discuss current critical questions in the field of biology
5. Develop communication, creative and critical thinking skills for life-long learning
a. Learn independently and then share that knowledge with others
b. Learn collaboratively and be willing to share expertise with peers
c. Demonstrate critical thinking skills such as analysis, discrimination, logical reasoning, prediction and transforming knowledge
d. Question the assumptions, sources, and contexts of scientific investigation
e. Demonstrate good observation skills and a curiosity about the world
6. Develop codes of social responsibility and scientific ethics, particularly in relation to biological advancement and applications
c. Respect regulations involving plagiarism and copyright
7. Demonstrate information literacy and technological fluency
a. Locate and evaluate information needed to make decisions, solve problems, design experiments, and understand scientific data
b. Work effectively with common technologies in biology
c. Evaluate and use biological databases (literature and public datasets)
Formative feedback: Lecturers and TAs will be closely working with you to monitor your learning progress. They will provide you with timely feedback to improve your technical skills and also provide you with suggestions for the experiments and data analysis. Furthermore, you will be given opportunities to express your ideas and discuss them with lecturers and TAs as course progresses. This will help you to achieve intended learning outcomes 1, 2, 3, 4, 5, 6, 7, 8 and 9.
You will be spending time to learn detailed biochemical principles of biological small molecules and macromolecules (water molecules, DNA/RNA, proteins, carbohydrates, lipids, vitamins) and related research skills and technological advances, which will help you to engage in molecular design initiatives (such as for biotechnology or drug development) and to conceive of ideas for designing new experiments and contemplating modern problems in biochemistry. To better engage you in class, we will make use of macromolecular graphics display and a mixture of modern and old school humour. In addition, you will be encouraged to ask questions or have discussions after the lecture.
TAs will provide materials containing concepts taught in classes and cover related applications derived from corresponding lectures. You will be assigned to a small group for interactive discussions, which will help you to develop your own critical thinking capability and problem solving skills.
You will have four lab sessions-- two of which are computer based and two of which are wet laboratory. You will acquire hands-on skills in analyzing biological macromolecules and also gain further insights into underlying molecular and chemical principles of proteins and carbohydrates. For the two lab sessions (wet and dry laboratory),you will be required to submit two written reports to evaluate your scientific data analysis skills, writing skills, and level of understanding on molecular principles.
Recommended textbook: Biochemistry, 4th Ed (2013), Mathews / Van Holde / Appling /Anthony-Cahill; ISBN-13: 978-0138004644
Recommended reference textbook: Lehninger Principles of Biochemistry, 5th Ed (2008), Nelson / Cox; ISBN: 9780716771081, W. H. Freeman, 2008
1. You are expected to read the lecture/tutorial/lab materials prior to the lecture/tutorial/lab session in question. This will help you to learn much more efficiently as you will already have an impression on the topics to be covered. You should also read through both of the two textbooks as outlined in the Weekly Schedule.
2. There are four laboratory sessions. The lab reports will be part of your continuous assessment.
Good academic work depends on honesty and ethical behaviour. The quality of your work as a student relies on adhering to the principles of academic integrity and to the NTU Honour Code, a set of values shared by the whole university community. Truth, Trust and Justice are at the core of NTU’s shared values.
As a student, it is important that you recognize your responsibilities in understanding and applying the principles of academic integrity in all the work you do at NTU. Not knowing what is involved in maintaining academic integrity does not excuse academic dishonesty. You need to actively equip yourself with strategies to avoid all forms of academic dishonesty, including plagiarism, academic fraud, collusion and cheating. If you are uncertain of the definitions of any of these terms, you should go to the Academic Integrity website for more information. Consult your instructor(s) if you need any clarification about the requirements of academic integrity in the course.
|Gruber, Ardina (Dr)||03s-48||6316 2926||Gruber@ntu.edu.sg|
|Week||Topic||Course ILO||Readings/ Activities|
Chemical Principles of Biochemistry
MVAA Ch 1, 2, 3
MVAA Ch 9
•Vitamins: a special class of biomolecules
MVAA Ch 19
|1, 2, 3, 5, 6|
MVAA Ch 5
|5, 8, 9|
MVAA Ch 6, 7,8
MVAA Ch 10
MVAA Ch 10
|2, 3, 4, 6|
MVAA Ch 4,
|2, 3, 4, 5, 6|
MVAA Ch 4
|2, 5, 6, 8|
MVAA Ch 4
|2, 5, 6, 8|
MVAA Ch 4
|1, 2, 3, 4, 5, 6, 7, 8, 9, 10|
You will be required to conduct two experimental sessions using Pymol software to analyse biomacromolecules in particular scenarios and two experimental sessions to detect/characterize proteins and sugars (each lab session carries equal weight and the four lab sessions will be scaled to contribute to 50% of the course total). You will need to interpret and analyse your results.
|Fail standard |
|Pass standard |
|High standard |
|1. Experimetal data report|| |
No correct data
|2. Data interpretation|| |
No explanations about the data
Lack clear explanation of the data.
|3. Further discussion|| |
No discussion or just superficial discussions
Lack deep discussions.
High quality further discussions
As a result of this course, it is expected you will develop the following "big picture" attributes:
Appreciate the roles of biological macromolecules (DNA, RNA, proteins, vitamins) in the life sciences
Appreciate the role of scientific discovery in transforming people's lives