Dr. Dhirendra Singh, PhD

University of Oulu

Biochemistry and Molecular Medicine
Finland

PhD Thesis:

Unravalling structural and mechanistic elements of catalytic subunits A and coupling subunit F and structural and kinetic studies of the A₃B₃D and A₃B₃DF-subcomplexes of the Methanosarcina mazei Gö1 provide insights into the single molecule dynamics of this engine. Degree in 2017

Publications:

1. Ramesh, B.D., Singh, D. and Biswas, S. (2013) GGD (/E) EF domain: a ubiquitous eubacterial domain confers cell toxicity. Int. J. Biotech. Res. 3, 73-80

2. Singh, D., Sielaff, H., Sundararaman, L., Bhushan, S. and Grüber, G.* (2016) The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase. Biochim. Biophys. Acta-Bioenergetics 1857, 177-187

3. Sielaff, H., Martins, J. Singh, D., Biuković, G., Grüber, G.*, Frasch, W.* (2016) Power Stroke Angular Velocity Profiles of Archaeal A-ATP synthase versus Thermophilic and Mesophilic F-ATP synthase Molecular Motors. J. Biol, Chem. 291, 25351-35363

4. Singh, D., Sielaff, H., Börsch, M.*, and Grüber, G.* (2017) Dynamics of subunit F in the A3B3DF-complex of Methanosarcina mazei Gӧ1 A-ATP synthase monitored by single-molecule FRET. FEBS Letters 591, 854-862

5. Ragunathan, P, Sielaff, H., Sundararaman, L., Biuković, G., Manimekalai, M.S.S., Singh, D., Kundu, S., Wohland, T., Frasch, W., Dick, T. and Grüber, G.* (2017) The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation. J. Biol. Chem. 292, 11262-11279

6. Singh, D. and Grüber, G.* (2018) Crystallographic and enzymatic insights into the mechanisms of Mg-ADP inhibition in the A1 complex of the A1AO ATP synthase. J. Struct. Biol. 201, 26-35

7. Sielaff, H., Singh, D., Grüber, G.* and Börsch, M.* (2018) Analyzing conformational changes in single FRET-labeled A₁ parts of archaeal A₁A_O-ATP synthase. Proc. SPIE 105007; DOI: 10.1117/12.2286785