Dr. Hendrik Sielaff, PhD

National University of Singapore

Publications:

  1. Nitsche, A., Kurth, A., Dunkhorst, A., Pänke, O., Sielaff, H., Junge, W., Muth, D., Scheller, F., Stöcklein, W., Dahmen, C., Pauli, G., and Kage, A. (2007) One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX. BMC Biotechnol. 7, 48

  2. Sielaff, H., Rennekamp, H., Wächter, A., Xie, H., Hilbers, F., Feldbauer, K., Dunn, S., Engelbrecht, S., and Junge, W. (2008) Domain compliance and elastic power transmission in rotary FOF1-ATPase. Proc. Natl. Acad. Sci. USA 105, 17760-17765

  3. Sielaff, H., Rennekamp, H., Engelbrecht, S., and Junge, W. (2008) Functional Halt Positions of Rotary FOF1-ATPase Correlated with Crystal Structures. Biophys. J. 95, 4979-4987

  4. Junge, W., Sielaff, H., and Engelbrecht, S. (2009) Torque generation and elastic power transmission in the rotary FOF1-ATPase. Nature 459, 364-370

  5. Rieger, R., Kobitski, A., Sielaff, H., and Nienhaus, G. U. (2011) Evidence of a Folding Intermediate in RNase H from Single-Molecule FRET Experiments. ChemPhysChem 12, 627-633

  6. Wächter, A., Bi, Y., Dunn, S. D., Cain, B. D., Sielaff, H., Wintermann, F., Engelbrecht, S., and Junge, W. (2011) Two rotary motors in F-ATP synthase are elastically coupled by a flexible rotor and a stiff stator stalk. Proc. Natl. Acad. Sci. USA 108, 3924-3929

  7. Sielaff, H., and Börsch, M. (2012) Twisting and subunit rotation in single FOF1-ATP synthase. Philos. T. R. Soc. B. 368, 20120024

  8. Hilbers, F., Junge, W., and Sielaff, H. (2013) The Torque of Rotary F-ATPase Can Unfold Subunit Gamma If Rotor and Stator Are Cross-Linked. PLoS ONE 8, e53754

  9. Ho, J.1, Sielaff, H.1, Nadeem, A., Svanborg, C. and Grüber, G. (2015) The molecular motor F-ATP synthase is targeted by the tumoricidal protein HAMLET. J. Mol. Biol. 427, 1866-1874

  10. Singh, D., Sielaff, H., Sundararaman, L., Bhushan, S. and Grüber, G.* (2016) The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase. Biochim. Biophys. Acta-Bioenergetics 1857, 177-187

  11. Sielaff, H., Martins, J. Singh, D., Biuković, G., Grüber, G.*, Frasch, W.* (2016) Power Stroke Angular Velocity Profiles of Archaeal A-ATP synthase versus Thermophilic and Mesophilic F-ATP synthase Molecular Motors. J. Biol, Chem. 291, 25351-35363

  12. Singh, D., Sielaff, H., Börsch, M.*, and Grüber, G.* (2017) Dynamics of subunit F in the A3B3DF-complex of Methanosarcina mazei Gӧ1 A-ATP synthase monitored by single-molecule FRET. FEBS Letters 591, 854-862

  13. Ragunathan, P, Sielaff, H., Sundararaman, L., Biuković, G., Manimekalai, M.S.S., Singh, D., Kundu, S., Wohland, T., Frasch, W., Dick, T. and Grüber, G.* (2017) The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation. J. Biol. Chem. 292, 11262-11279

  14. Sielaff, H., Singh, D., Grüber, G.* and Börsch, M.* (2018) Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase. Proc. SPIE 105007; DOI: 10.1117/12.2286785

  15. Bogdanović, N., Trifunovic, D., Sielaff, H., Westphal, L., Bhushan, S., Müller, V. and Grüber, G. (2019) The structural features of Acetobacterium woodii F-ATP synthase reveal the importance of the unique subunit γ-loop in Na+ translocation and ATP synthesis. FEBS J. 286, 1894-1907

1 (Authors have equal contribution)

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last update 23-Nov-2020