Dr. Youg Raj Thaker, PhD

Harvard Medical School

Sharpe Laboratory

Department of Microbiology and Immunology

77 Avenue Louis Pasteur, NRB 837

Boston, MA 02115.

PhD Thesis:

Caspase interaction of antiapoptotic Livin as well as the vacuolar ATPase (V- ATPase) and structural insight into the subunits a and d of the yeast V-ATPase. Degree in 2009

Publications:

1. Thaker, Y.R. and Bajaj, B.K. (2006) Antibiotic resistance and molecular characterization of poultry isolates of Salmonella by RAPD-PCR. World J. Microbiol. Biotechnol. 22, 1177-1183

2. Thaker, Y. R., Rössle, M. and Grüber, G. (2007) The boxing glove shape of subunit d of the yeast V-ATPase in solution and the importance of disulfide formation for folding of this protein. J. Bioenerg. Biomembr. 39, 275-89

3. Rishikesan, S., Thaker, R. Y., Priya, R., Gayen, S., Manimekalai, M. S. S., Hunke, C. and Grüber, G. (2008) Spectroscopical identification of residues of subunit G of the yeast V-ATPase in its connection with subunit E. Mol. Mem. Biol. 25, 400-410

4. Rishikesan, S., Gayen, S., Thaker, R. Y., Vivekanandan, S., Manimekalai, M. S. S., Yau, Y. H., Greifman Shochat, S. and Grüber, G. (2009) Assembly of subunit d (Vma6p) and G (Vma10p) and the NMR solution structure of subunit G (G1-59) of the Saccharomyces cerevisiae V1VO ATPase. Biochim. Biophys. Acta-Bioenergetics 1787, 242-251

5. Thaker, R. Y.¹, Hunke, C.¹, Yau, Y. H., Greifman Shochat, S., Ying, L. and Grüber, G. (2009) Association of the eukaryotic V1VO ATPase subunits a with d and d with A. FEBS Lett. 583, 1090-1095

6. Merkulova, M. Bakulina, A., Thaker, Y. R., Grüber, G. and Marshansky, V. (2010) Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO. Biochim. Biophys. Acta-Bioenergetics 1797, 1398-1409

7. Basak, S., Gayen, S., Thaker, Y. R., Manimekalai, M. S. S., Roessle, M., Hunke, C., and Grüber, G. (2011) Solution structure of subunit F (Vma7p) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae derived from SAXS and NMR spectroscopy. Biochim. Biophys. Acta-Biomembranes 1808, 360-368

8. Rishikesan, S., Thaker, Y. R., and Grüber, G. (2011) NMR Solution Structure of Subunit E (fragment E1-69) of the Saccharomyces cerevisiae V1VO ATPase. J. Bioenerg. Biomembr., 43, 187-193

9. Marshansky, V., Hosokawa, H., Merkulova, M., Bakulina, A., Bjargava, A., Tonra, J., Dip, P.V., Thaker, Y.R., and Grüber, G. (2019) Structural model of a2-subunit N-terminus and its binding interface for Arf-GEF CTH2: Implication for regulation of V-ATPase, CTH2 function and rational drug design. Curr. Top. Membr. 83, 77-106

¹ (Authors have equal contribution)