Dr. Saw Wuan Geok, PhD

Nanyang Technological University

NTU Institute of Structural Biology
59 Nanyang Drive
Singapore 636921

FYP Thesis:

Cloning, Expression, and Purification of Nucleotide-binding Domain 1 (NBD1) of Multidrug Resistance Protein (PfMDR1) from Plasmodium falciparum. Degree in 2012

PhD Thesis:

Solution studies on the Dengue virus Non-structural protein 5 and -3 as well as the Capsid protein. Degree in 2017

Publications:

  1. Dip, P. V.1, Saw, W. G.1, Roessle, M., Marshansky, V., and Grüber, G. (2012) Solution structure of subunit a, a104-363, of the Saccharomyces cerevisiae V-ATPase and the importance of its C-terminus in structure formation. J. Bioenerg. Biomembr. 44, 341-350

  2. Saw, W. G., Eisenhaber, B., Eisenhaber, F., and Grüber, G. (2013) Low resolution structure of the soluble domain GPAA1 (yGPAA170-247) of the glycosylphosphatidylinositol transamidase subunit GPAA1 from Saccharomyces cerevisiae. Biosci. Rep. 33, e00033

  3. Dip, P. V.1, Kamariah, N.1, Nartey, W.1, Beushausen, C., Kostyuchenko, V. A., Ng, T. S., Lok, S. M., Saw, W. G., Eisenhaber, F., Eisenhaber, B. and Grüber, G. (2014) Key roles for the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress. Biochim. Biophys. Acta 1837, 1932-1943

  4. Tay, M. Y. F., Saw, W. G., Zhao, Y., Chan, W. K. K., Singh, D, Chong, Y., Forwood, J. K., Ooi, E. E., Grüber, G., Lescar, J., Luo, D., and Vasudevan, S. G. (2015) The C-terminal 50 amino acid residues of Dengue NS3 protein are important for NS3-NS5 interaction and viral replication. J. Biol. Chem. 290, 2379-2394

  5. Saw, W. G., Tria, G., Grüber, A., Manimekalai, M. S. S., Zhao, Y., Chandramohan, A., Anand, G. S., Matsui, T., Weiss, T., Vasudevan, S. and Grüber, G. (2015) Structural insight and flexibility features of NS5 proteins from all four serotypes of Dengue virus in solution. Acta Crystallogr. D71, 2309-2327

  6. Manimekalai, M.S.S., Saw, W. G., Pan, A., Grüber, A. and Grüber, G. (2016) Identification of the critical linker residues conferring differences in compactness of DENV-4 NS5 from Dengue virus serotypes 1-3. Acta Crystallogr. D72, 795-807

  7. Saw, W. G.,1 Pan, A.,1 Manimekalai, M.S.S. and Grüber, G.* (2017) Structural features of Zika virus non-structural proteins 3 and -5 and its individual domains in solution as well as insights into NS3 inhibition. Antiviral Res. 141, 73-90

  8. Zhu, G., Saw, W. G., Nalaparaju, A., Grüber, G. and Lu L. (2017) Coarse-Grained Molecular Modeling of Solution Structure Ensemble of Dengue Virus Non-Structural Protein 5 with Small-Angle X-Ray Scattering Intensity. J. Phys. Chem. B. 121, 2252-2264

  9. Pan, A.1, Saw, W.G.1, Manimekalai, M.S.S., Grüber, A., Shin, J., Matsui, T., Weiss, T. and Grüber, G.* (2017) Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin. Acta Crystallogr. D73, 402-419

  10. Wong, C.F., Shin, J., Manimekalai, M.S.S., Saw, W.G., Zhan, Y., Bhushan, S. Kumar, A., Ragunathan, P. and Grüber, G.* (2017) The uniqueness of AhpC of the mycobacterial antioxidant defense system and its interaction with its reducing partner Thioredoxin-C. Sci. Rep. 7, 5159

  11. Boon, P.L.S.1, Saw, W.G.1, Lim, X.X.1, Raghuvamsi, P.V., Huber, R.G., Marzinek, J.K., Holdbrook, D.A., Ganesh, S.A., Grüber, G.* and Bone, P.J. (2018) Partial Intrinsic Disorder Governs the Dengue Capsid Protein Conformational Ensemble. ACS Chem Biol. DOI: 10.1021/acschembio.8b00231

  12. Saw, W.G.1, Pan, A.1, Manimekalai, M.S.S., Grüber, A., and Grüber, G.* (2018) Structure and flexibility of Non-structural proteins 3 and -5 of Dengue- and Zika viruses in solution. Progr. Biophys. Mol. Biol. 143, 67-77

  13. Ng, I.H.W., Chan, K., Tan, M.J.A., Gwee, C.P., Smith, K.M., Jeffress, S.J., Saw, W.G., Swarbrick, C.M.D., Watanabe, S., Jans, D., Grüber, G., Forwood, J.K. and Vasudevan, S.G. (2019) Zika virus NS5 forms supramolecular nuclear bodies that sequester importin alpha and modulate the host immune and pro-inflammatory response in neuronal cells. ACS Infect. Dis. 2019, 5, 932-948

  14. Saw, W.G., Chan, K., Vasudevan, S.G. and Grüber, G.* (2019) Zika virus non-structural protein 5 residue 681 is critical for dimer formation and enzymatic activity. FEBS Lett. 593, 1272-1291

  15. Saw, W.-G.1, Wu, M.-L1, Ragunathan, P.1, Biuković, G, Lau, A.-M., Shin, J., Harikishore, A., Cheung, C.-Y., Hards, K., Sarathy, J. P., Bates, R. W., Cook, G. M. C., Dick, T.*, and Grüber, G.* (2019) Disrupting coupling within mycobacterial F-ATPsynthase subunit ε causes dysregulated energy production and cell wall biosynthesis. Sci. Rep. 9, 16759

  16. Kamariah, N., Ragunathan, P., Shin, J., Saw, W.-G., Wong, C.F., Dick, T., and Grüber, G.* (2020) Unique structural and mechanistic properties of mycobacterial F-ATP synthases: Implications for drug design. Prog. Biophys. Mol. Biol. 152, 64-73

  17. Saw, W.-G., Wong, C.-F., Dick, T., Grüber, G.* (2020) Overexpression, purification, enzymatic and microscopic characterization of recombinant mycobacterial F-ATP synthase. Biochem. Biophys. Res. Comm. 522, 374-380

  18. Hotra, A., Ragunathan P., Ng, P.S., Seankongsuk, P., Harikishore, A., Sarathy, J.P., Saw, W.-G., Lakshmanan, U., Sae-Lao, P., Kalia, N.P., Shin, J., Kalyanasundaram, R., Anbarasu, S., Parthasarathy, K., Pradeep, C.N., Makhija, H., Dröge, P., Poulsen, A., Tan, J.H.L., Pethe, K., Dick, T., Bates, R.W., and Grüber, G.* (2020) Discovery of a novel Mycobacterial F-ATP synthase inhibitor and its potency in combination with diarylquinolines. Angewandte Chemie i.Ed, 132, 13397-13406

  19. Wong, C.-F., Lau, A.-M., Harikishore, A., Saw, W.-G., Shin J., Ragunathan, P., Bhushan, S., Ngan, S. F. C., Sze, S. K., Bates, R.W., Dick, T., and Grüber, G.* (2021) A systematic assessment of mycobacterial F1-ATPase subunit ε’s role in latent ATPase hydrolysis. FEBS J. 288, 818-836

  20. Qiao, Z., Lampugnani, E., Yan, X.-F., Khan, G., Saw, W.G., Hannah, P., Qian, F., Calabria, J. Miao, J., Grüber, G., Persson, S., and Gao, Y.G. (2021) Crystal structure of Arabidopsis CESA3 catalytic domain with UDP-Glucose provides insight into the mechanism of cellulose synthesis. Proc. Natl. Acad. Sci. USA 118, e2024015118

  21. Harikishore, A., Saw, W.-G., Ragunathan, P., Litty, D., Dick, T., Müller, V., and Grüber, G.* (2022) Mutational analysis of mycobacterial F-ATP synthase subunit δ leads to a potent δ enzyme inhibitor. ACS Chem. Biol. 17, 529-535

  22. Saw, W.-G., Leow, C.Y., Harikishore, A., Shin, J., Cole, M., Aragaw, W.W., Ragunathan, P., Hegde, P., Aldrich, C.C., Dick, T., and Grüber, G.* (2022) Structural and mechanistic insights into Mycobacterium abscessus aspartate decarboxylase PanD and a pyrazinoic acid-derived inhibitor. ACS Infect. Dis., in press

1 (Authors have equal contribution)

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last update 10-Jun-2022